Review on 🧤 Microflex Medical MidKnight MK-296-XL X-Large Black 9.645'' Nitrile Gloves, 4.7 mil, Non-Sterile, Medical Grade, Powder-Free, Disposable, English, 15.34 fl. oz., Plastic, 1" x 9.6" x 5.5 by Katie Miller

<p>Storage: -20°C UNSPSC code: 41105600 Ingredients: enzyme solution; SuRE/Cut Buffer A 10x concentrated RIDADR: NONH for all modes of transport. % •L: 75-100% •M: 50-75% General Description: Isoschiisomers Nar I is an isoschizomer of Bbe I, Ehe I, Kas I, Nun II. NarI methylation sensitivity is indicated by the presence of 5-methylcytosine the position marked with (*) in the recognition sequence is inhibited. In addition, Nar I is inhibited when 4-methylcytosine occurs at the 3' end of C and when 5'-hydroxymethylcytosine occurs at any of the three Cs. Ligation and recut assay NarI fragments obtained by complete digestion of 1 µg Ad2 DNA were ligated 1 unit. T4 DNA ligase in a volume of 10 µl by incubation for 16 hours at +4°C in 66mM Tris-HCl, 5mM MgCl 2 , 5mM dithiothreitol, 1mM ATP, pH 7.5 (at + 20°C), resulting in &gt;90% extraction of Ad2 DNA. Subsequent trimming with Nar I yields &gt;90% of the typical pattern of Ad 2 × Nar I fragments Other Notes: For Life Science Research Only. Not for use in diagnostic procedures. Quality: no unspecific endonuclease activity. 1 µg Ad2-DNA is incubated in 50 µl SuRE/Cut Buffer A with an excess of Nar I for 16 hours. The number of enzyme units that do not change the pattern characteristic of the enzyme is stated on the certificate of analysis. . No exonuclease activity Approximately 5 μg of [ 3 H]-labeled calf thymus DNA was incubated with 3 μl of NarI for 4 hours at +37°C in a total volume of 100 μl of 50 mM Tris-HCl, 10 mM MgCl 2 , 1 mM dithioerythritol, pH -value approx. </p>