No, I have not used it on puppies. I use super glue a lot (but not for puppies) and this is one of my favorite brands. It has a relatively long shelf life (e.g. 2 months above average) so you don't have to use it all at once. It's thick enough not to bleed and thin enough not to stick together when glued together. But use it with care or you'll stick your fingers together. Or accidentally tape your siblings together. Or you are brothers and sisters to your neighbors. But never puppies, because no one would dare glue puppies.