Follow the instructions after reading and watching the video. After 2.5 hours the CO2 content did not change. Added 1 teaspoon of yeast and 2 cups of sugar to 1 liter of warm water in a 2 liter soda bottle with holes in the lid and after 30 minutes my CO2 monitor was at its maximum and has been for the past 2 days (max 8000ppm). This DIY solution costs about $7 and works better. This is for my son's science fair project so I'm sure the DIY could probably be done better. >8000ppm seems pretty high. But we just need some data :). TNB Naturals was quick to answer my questions about how I set this up. I just replied so let's see what they have to say. UPDATE: TNB is sending me another one for free. Will be submitted as soon as it arrives
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