I used them when I was away for 45 minutes. These are small bags so I kept most of the fish separate except for 2-3 small schooling fish in the bag. I poured about 1.5 glasses of water and used a battery powered air pump to make sure they had enough oxygen. Rubber bands to seal, and they went to the fridge with a towel. Including disassembling the tank, cleaning, transporting and reloading the tank, the fish were probably in the bags for around 3-4 hours. Everything worked great, there were no leaks. Some bags lost some air, but it may have been my fault for not tying them tight enough.
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