Align and tighten once. If you need to loosen them, the coating on the bolt and nut will cause the threads to seize and the nut to freeze onto the bolt. I ended up taking the tire off the plastic, shearing off the bolts and replacing the bolts and fasteners with grade 5 stainless steel. Also, I had a twist/twist on the stud in the plastic, so I would recommend you take the tire off the plastic, tighten the screws and reassemble.
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