We ran 2 aquariums in less than 5 days each. We took samples to our local fish shop and they were surprised. We had 2 10 gallon tanks for my son's first pets. We bought most of the equipment for them in the store so they knew how long it took to set everything up. I'm not sure they would have believed us otherwise. They don't currently have a bacterial nitrogen cycle product, but I suspect we'll find one on their shelves soon. (They thought we were just throwing our money away when I asked about the product for it.) Important note: This is a living product and will quickly poison an entire aquarium if not fresh. I tested the contents for nitrogen before putting them in a livestock tank. The test is simple and quick, so it doesn't matter. If you don't use your entire stash, store the rest in a cool, dry place, then test before using in a live animal environment. Good product. We also tried the Tetra bacteria package, but it didn't affect the nitrogen in the tank despite waiting several days.
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